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Trypan Blue Solution

Trypan Blue Solution Specification

Usage Type
Laboratory/Research

Accuracy
High for Viability Differentiation

Function
Viable Cell Exclusion by Staining

Shelf Life
24 Months

Features
Ready-to-use, High Purity, Non-Sterile

Instruments Type
Biological Reagent

Storage Instructions
Store at Room Temperature, Away from Light

Equipment Type
Biological Stain Solution

Material
Aqueous Solution

Condition
New

Technology
Laboratory Grade Chemical

Portable
Yes

Wall Mounted
No

Real-Time Operation
No

Operating Type
Manual

Use
Hospital

Dimension (L*W*H)
Varies by Packaging (e.g. 30 ml bottle)

Weight
Variable (depends on packing, e.g. 50 g for 30 ml bottle)

Color
White

Hazardous Status
Non-Hazardous for Transport

Grade
Laboratory/Research Grade

Application
Microscopy, Cell Counting, Dye Exclusion Assays

Expiration Date
As mentioned on bottle

Chemical Formula
C34H24N6Na4O14S4

CAS Number
72-57-1

pH Range
6.5-7.5

Packaging Type
Plastic Bottle with Screw Cap

Concentration
0.4% (w/v) in Aqueous Solution

Solubility
Water Soluble

About Trypan Blue Solution

Trypan Blue Solution offered to our customers, is an acid azo dye commonly used as a stain to distinguish viable from non-viable cells. It turns dead cells blue and viable cells unstained. It is a known animal carcinogen and an experimental teratogen. This solution is a cell impermeant stain used to estimate the number of dead cells in a viable population. This solution can be easily purchased by our clients at nominal prices.

High Accuracy in Cell Viability Assays

With a concentration of 0.4%, Trypan Blue Solution ensures precise differentiation between viable and non-viable cells in applications such as microscopy, cell counting, and dye exclusion assays. Its high purity and laboratory-grade standard make it a dependable choice for research professionals.

Convenient & Versatile Packaging

Packaged in a robust plastic bottle with a secure screw cap, this biological stain is easy to use and portable. The packaging options vary, allowing flexibility for different laboratory scales, while ensuring stability, safety, and ease of handling during transport and storage.

Long Shelf Life & Simple Storage

Trypan Blue Solution offers a shelf life of 24 months when stored at room temperature and protected from light. Its aqueous formulation ensures high solubility and stability, supporting long-term laboratory or hospital use without the need for cold storage.

FAQ's of Trypan Blue Solution:

Q: How is Trypan Blue Solution typically used in laboratories?

A: Trypan Blue Solution is primarily used for cell viability assays. It allows researchers to distinguish between viable and non-viable cells under a microscope by selectively staining dead cells, making it a key reagent in cell counting and dye exclusion assays.

Q: What are the storage requirements for this product?

A: Store the Trypan Blue Solution at room temperature, away from direct light, to preserve its efficacy and maintain the 24-month shelf life indicated on the packaging. Refrigeration is not required.

Q: When should the solution be replaced or discarded?

A: The solution should be replaced or discarded once it has exceeded the expiration date mentioned on the bottle, or if there is any sign of contamination or discoloration that deviates from the expected white appearance.

Q: Where can Trypan Blue Solution be used?

A: This product is suitable for use in hospitals, research laboratories, and other scientific settings requiring accurate cell viability analysis. It is designed for indoor use and not intended for wall mounting or automated systems.

Q: What is the process for applying Trypan Blue in cell counting?

A: To perform cell counting, mix a small volume of Trypan Blue Solution with a cell suspension, incubate briefly, and then examine the mixture under a microscope. Non-viable (dead) cells will uptake the dye and appear blue, while viable cells remain clear.

Q: What are the main benefits of using this Trypan Blue Solution?

A: The main benefits include high accuracy for cell viability studies, ready-to-use convenience, water solubility, and compliance with laboratory-grade standards. It's also non-hazardous for transport, making storage and handling safe and easy.

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